Xylotrophic Agaricomycetes – Antagonists Of Rare Mushroom Hericium Coralloides (scop.) Pers. (hericiaceae) In The Pure Culture
Abstract
M. V. Pasaylyuk
We established the patterns of growth and type of mutual relations of Hericium сoralloides 2332 culture from PDA with the presence cultures of Fomes fomentarius 1528 and/or Schizophyllum commune 1763. It is established that cultures of F. fomentarius, і S. commune are antagonists to H. сoralloides on the PDA by direct confrontation and consecutive combined cultivation culture method. But, during direct confrontation mycelium of H. сoralloides cultivation with culture F. fomentarius and/or S. commune the inhibition effect was 60-84% on the 10th day of the experiment with stopping growth of H. сoralloides. In the case of consecutive combined cultivation culture method, the inhibition effect was 15-35%. Mycelium of H. сoralloides was growing to contact with antagonists. The obtained results indicate the importance for the growth and survival of H. coralloides mycelia of primary occupation of the nutrient medium in binary and triple compositions. Between H. coralloides and F. fomentarius for direct confrontation the fact of bilateral antagonism is established. For the triple composition of the selected cultures, the phenomenon of additive antagonism is characterized. For cultures of the composition the S. commune + F. fomentarius on the 10th day of the experiment, the fact of equilibrium confrontation was established. In direct confrontation for composition H. coralloides + F. fomentarius colonial characteristic was bilateral growth inhibition at a distance from the formation of inhibition zones. Colonies from binary composition F. fomentarius + S. commune and triple H. coralloides + F. fomentarius + S. commune for direct confrontation growth stopped after contact. For variant H. coralloides + S. commune growth has been a stop of the colonies H. coralloides followed the growth of mycelium S. commune. In a series of consecutive combined cultivation culture method for binary and triple composition of cultures after contact the colonies of H. coralloides growth stopped, rare mushroom the colony continued to grow over antagonist’s mycelium, but never overgrown it completely. According to the growth coefficients on PDA the monocultures S. commune and F. fomentarius belong to those growing at an average speed under the given experimental conditions, H. coralloides culture is slowly growing. These results are important for the remediation H. coralloides in vivo to avoid the loss of seed inoculum of H. coralloides. Fact antagonism between H. coralloides and F. fomentarius must be viewed for logs Fagus silvaticus involved to remediation H. coralloides.
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