The evaluation of safety of broiler chickens’ meat with organoliptic indicators and it’s toxity after the use of fluoroquinolone antibiotics

Abstract

S.A. Tkachuk, V.B. Dukhnitsky, V.P. Lyasota, I.V. Yazenko, I.V. Zabarna, L.B. Savchuk, N.P. Holovko, S.B. Prosianyi, A.B. Mushynskyi, N.V. Usachenko

The negative effect of the residual amounts of antibiotics in poultry slaughter products on human health is that they cause sensitizing effects, the risk of allergic reactions, may cause a dysbiosis, superinfections, the formation of resistant strains of pathogenic microorganisms and a decrease in the therapeutic effectiveness of antibiotics. Antibiotics worsen the sanitary qualities and technological properties of a poultry meat; distort the results of bacterial insemination. The article deals with the experimental application of the Danoksan-50 antibacterial preparation for broiler chickens of the Cobb-500 crossbreed of a slaughter age. Danofloxacin is a broad-spectrum chemotherapeutic drug that belongs to the 4th generation of fluoroquinolone antibiotics group. It is active against gram-positive and gram-negative microorganisms, mycoplasmas. The influence of fluoroquinolone antibiotics on the microbiological parameters and toxicity of femoral and pectoral muscles of broiler chickens is not sufficiently studied. Therefore, the aim was to study the influence of Danoksan-50 on the safety of broiler chickens’ meat. The use of fluoroquinolone antibiotics for broiler chickens do not affect the level and species composition of microflora in the studied samples of poultry, as all the microbiological parameters in experimental groups meet the requirements of current regulations. Basing on microbiological indicators, it was found that the quantity of mesophilic aerobic and facultative anaerobic microorganisms (QMAFAnM), Escherichia coli bacteria, Proteus, Salmonellа and S. аureus, L. monocytogenes bacteria in femoral and pectoral muscles of broiler chickens from 24 to 120 hours, after the last oral feeding with Danoksan-50, does not exceed the permissible levels. According to the toxico-biological assessment results, using Tetraсhуmena pуriformis ciliates, in pectoral and femoral muscles in 24 hours – after the last use of Danoksan-50 in a dose of 0.1 ml/kg of a body weight for 5 days – no toxic effects on broiler chickens’ meat have been found; and that is confirmed with a ciliates death rate of 0.2-1.0% and an increase in their total number.

Keywords: Broiler chickens; Danoksan-50; microbiological indicators; toxicity

References:
Krysanov, D. (2010). Yakist i bezpechnist kharchovoi produktsii. Ekonomika i prohnozuvannia. 3, 103-119. Available from http://nbuv.gov.ua/UJRN/econprog_2010_3_11.
Iakubchak, O. N., Zabarna, I. V., Taran, T. V. (2017). Effect of Farmazin® and Tilocyclinvet® on microbiological, chemical, and microscopic characteristics of slaughtering products of broiler chickens. Ukrainian Journal of Ecology, 7(4), 125-133. (in Ukrainian). doi: 10.15421/2017_95.
Kasianenko, О., Fotina, Т., Proshyna, А., Sobyna, М., Fotina, Н. (2015). Kontrol mikrobiolohichnoi bezpeky produktsii ptakhivnytstva. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia: Veterynarna medytsyna, 7, 85-89. Available from: http://nbuv.gov.ua/UJRN/Vsna_vet_2015_7_25.
Fotina, T. (2007). Riven vmistu antybakterialnykh preparativ u produktakh kharchuvannia ta syrovyni tvarynnoho pokhodzhennia. Nauk. tekhn. biul. in-tu biolohii tvaryn i DNKI vetpreparativ ta kormovykh dobavok. 3(4), 107-110.
Kaniuka, O., Pavliv, О. (2009). Ekolohichni problemy zalyshkovykh kilkostei antybiotykiv u produktakh tvarynnytstva. Naukovyi visnyk. 11(3), 39-42.
Holovko, A. (2014). Monitorynh zalyshkiv protymikrobnykh preparativ u produktsii tvarynnytstva riznykh vyrobnykiv ta osnovni prychyny zabrudnennia. Veterynarna medytsyna Ukrainy. 5, 28-30.
Bokma-Bakker, M. (2014). Antibiotic use in Brazilian broiler and pig production: an indication and forecast of trends. Wageningen UR Livestock Research. 714, 1-25.
Yamaguchi, T. (2015). Antibiotic residue monitoring results for pork, chicken, and beef samples in Vietnam in 2012–2013. Journal of agricultural and food chemistry. 63(21), 51415145. doi: 10.1021/jf505254y.
Offiah, N. (2015). Detection of Antimicrobial Residues in Chicken Muscle and Liver Sold at Retail Outlets in Trinidad. International Journal of Poultry Science. 14(8), 456. doi: 10.3923/ijps.2015.456.462.
Goudah, A., Mouneir, S. (2009). Disposition kinetics and tissue residues of danofloxacin in Muscovy ducks. British poultry science. 50(5), 613-619. doi.org/10.1080/00071660903147416.
Er, B., Onurdag, F. K., Demirhan, B., Ozgacar, S. Ö., Oktem, A. B., Abbasoglu, U. (2013). Screening of quinolone antibiotic residues in chicken meat and beef sold in the markets of Ankara, Turkey. Poultry science, 92, 2212-2215. doi: 10.3382/ps.2013-03072.
Muylaert, A., Mainil, J. G. (2013). Quinolones and fluoroquinolones: decades of development and use. Annales de Médicine Vétérinaire, 157(2), 72-81.
Yevropeiska konventsiia pro zakhyst khrebetnykh tvaryn, yakykh vykorystovuiut dlia eksperymentalnykh ta naukovykh tsilei. (1995). Available from: http://zakon.nau.ua/doc/?code=994_137.
Mjaso pticy. Subprodukty i polufabrikaty ptich'i. Metody otbora i podgotovka prob k mikrobiologicheskim issledovanijam: GOST 7702.2.0–95. [Data vvedenija 1997-01-01]. (2000). M.: Iz-vo standartov, 4, 284.
Produkty pishhevye. Metod opredelenija kolichestva mezofil'nyh ajerobnyh i fakul'tativno-anajerobnyh mikroorganizmov: GOST 10444.15–94. [Data vvedenija 2010-26-05]. (2010). M.: Iz-vo standartov, 170.
Mikrobiologija. Obshhee rukovodstvo po podschetu predpolagaemoj Escherіchіa colі: DSTU ISO 7251:2006. [Data vvedenija 29.06.2006]. (2006). K.: Iz-vo standartov, 28.
DSTU ISO 6579:2006 Mikrobiolohiia kharchovykh produktiv i kormiv dlia tvaryn. Metodyka vyiavlennia Salmonella spp. [Data vvedenyia 01.10.2008]. (2008). K.: Yz-vo standartov, 28.
DSTU ISO 11290-1:2003. Mikrobiolohiia kharchovykh produktiv ta kormiv dlia tvaryn. Horyzontalnyi metod vyiavlennia ta pidrakhovuvannia Listeria monocytogenes. Ch. 1: DSTU ISO 11290-1:2003; Metod vyiavlennia (ISO 11290-1:1996, IDT). per. i nauk.-tekhn. red. M. Rozhko [ta in.]. Chynnyi vid 01.10.2004. [B. m.]. (2005). IV, 18.
Mjaso pticy, subprodukty i polufabrikaty ptich'i. Metody vyjavlenija bakterij roda Proteus. M’jasna produkcіja ta jajce-produkti: GOST 7702.2.7-95. [Data vvedenija 1996-01-01]. (2000). 4, 284.
GOST 10444.2–94. Produkty pishhevye. Metody vyjavlenija i opredelenija kolichestva Staphylococcus aureus. (1996). 11.
Mykytiuk, P., Bukalova, N., Dzhmil,V. (2004). Metodychni vkazivky (mikrometod) shchodo vykorystannia infuzorii Tetrakhimena piriformis dlia toksyko-biolohichnoi otsinky silskohospodarskykh produktiv ta vody. Bila Tserkva, 22.
Nakaz Derzhavnoho departamentu veterynarnoi medytsyny vid 3 lystopada 1998 roku No 16 «Pro zatverdzhennia Obovʼiazkovoho minimalnoho pereliku doslidzhen syrovyny, produktsii tvarynnoho ta roslynnoho pokhodzhennia, kombikormovoi syrovyny, kombikormiv, vitaminnykh preparativ ta in., yaki slid provodyty v derzhavnykh laboratoriiakh vetmedytsyny i za rezultatamy yakykh vydaietsia vetsvidotstvo (F-2)». (2004). 112.
 

<

Share this article