Immunohistochemicals Characteristic Of Subpopulations Of Pigeon And Chicken Splenic Lymphocytes
Abstract
O.F. Dunaievska
The article is devoted to the study of immunohistochemical features of the spleen of birds based on the detection and morphometric calculation of clusters of the T- and B-lymphocytes. For the research, the selection of the spleen is performed at the stage of morphofunctional maturity in clinically healthy sexually mature birds of both sexes in the ratio 1: 1: blue pigeons (age 10-14 months), chickens of domestic breeds of Poltava clay (age 19-20 weeks). The slices of material are fixed in 10-12% chilled solution of neutral formalin, embedded in paraffin. Histological sections made on a microtome MS-2 and have a thickness not exceeding 5 micrometres. The mouse monoclonal antibodies of the Danish firm DAKO are used to detect and study subpopulations of lymphocytes. Morphometric studies are performed under light microscopy (Microscope Biolam-Lomo and Micros MC-50). The microphotography of histological preparations was carried out with CAM V 200 video camera. It is mounted on a microscope Micros MC-50. Subpopulations of lymphocytes CD4 +, CD8 +, СD19 +, СD20 + were detected in the red and white pulp of the spleen of pigeons and chickens, they are situated singly and diffusely, more often formed chains or aggregations. The CD4 + cells in pigeons and chickens are dominated in lymphoid sheaths near the vessels, amounted the 56.63 ± 4.03 pieces per conditional unit of area and 85.71 ± 6.09 pieces per conditional unit of area respectively. The populations of CD8 + cells are also predominated in lymphoid sheaths near the vessels, but their number was smaller than the CD4+ population by 1.81 times in chickens and by 2.36 times in pigeons. In the red pulp, CD4 + cells are occupied 7.67% of the total population of the splenic cells in chickens and 5.99% in pigeons. The CD8 + cell in the red pulp are less concentrated: 2,8% and 4,45% respectively. The index of immunoregulation of the spleen of chickens was 1.85, and the pigeons – 2.0. B-lymphocytes (cluster CD19 +, CD20 +) are concentrated in the B-dependent zone of the spleen – the lymphoid nodules. Most of the CD20 + cells are in lymphoid nodules of chickens (77.27%), in pigeons this indicator is 61.66%. Cells of CD19 + in lymphoid nodes are occupied approximately the same relative square of pigeons and chickens from their total amount in the pulp – 74.14% and 76.60%. The total number of T-lymphocytes (the population of CD4 +, CD8 +) are prevailed almost by two times in chickens, B-lymphocytes (populations CD19 +, CD20 +) in pigeons – by 1,6 times. In the spleen of pigeons and chickens are developed both humoral and cellular immune protection.
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