Effects Of Microelements On The Carotenoid Synthesis By Some Basidiomycetes Strains
Abstract
A. K. Velygodska, O. V. Fedotov
The effect of microelements on growth and accumulation of carotenoids highly productive strains of basidiomycetes at surface cultivation on glucose-peptone medium was investigated. The objects of research are 3 wood destroying strain. There are Laetiporus sulphureus (Bull.) Murrill Ls-08, Fomes fomentarius (L.) Fr. Ff-1201 from the order Polyporales and Fistulina hepatica (Schaeff.) Sibth Fh-18 from the order Agaricales. Research materials are strains mycelium and culture filtrate (CF). Absolutely dry biomass (ADB) mycelium was determined by the gravimetric method, the content of carotenoids was determined by spectrophotometric method in acetone extracts of the Vetshteyn formula. Established individual influence of microelements on the accumulation of biomass and carotenoids of basidiomycetes strains. The possibility of the regulation of these processes by introducing into the glucose-peptone medium of various Fe, Cu, Zn, Ni and Mn sulphate. So, the best to increase the intensity of the growth processes and the accumulation of carotenoids strain of L. sulphureus Ls-08 is an experimental environment which includes Zn sulfate in a concentration of 8 mmol/L. To induce the accumulation of ADB and carotenoids in the mycelium and CF of strain F fomentarius Ff-1201 making in is expedient Mn sulfate in a concentration of 1.6 mmol/L. To improve carotenogenesis of F. hepatica Fh-18 strain expedient entry in GPM Mn sulphate at concentration of 8 mmol/L. These allow to optimize the concentration of microelements in nutrient medium for the cultivation of carotenoids high-producing strains of Basidiomycetes.
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